By Weiming Xia, Huaxi Xu
Within the look for an efficient remedy for Alzheimer's illness, APP is a special version protein that illustrates the big variety of simple and complex characterization strategies to be had.
Exploring numerous organic thoughts to explain the constitution and serve as of this transmembrane protein, this article provides each one procedure with targeted, step by step protocols to accomplish reproducible effects and supply a framework for learning different membrane proteins.
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Extra resources for Amyloid precursor protein : a practical approach
H. Trafficking of cell surface beta-amyloid precursor protein: retrograde and transcytotic transport in cultured neurons. J. Cell Biol. 129, 431, 1995. 23. A. A. Rab15 mediates an early endocytic event in Chinese hamster ovary cells. J. Biol. Chem. 274, 22303, 1999. fm Page 29 Wednesday, November 3, 2004 2:59 PM 3 Strategies for Crystallizing the N-Terminal Growth Factor Domain of Amyloid Precursor Protein William J. McKinstry, Susanne C. Feil, Denise Galatis, Roberto Cappai and Michael W. 6 Expression of Recombinant GFD in Pichia pastoris.
To calculate the specific binding, the radioactivity from untransfected control cells is subtracted from the counts obtained from the transfected cells in each condition. The results obtained at each time point are expressed as a percentage of total radioactivity from the three fractions (medium, acid wash and cell lysate). The anticipated result should show a rapid release of sAPP into the medium with a half life of 10 min. Consequently, cell surface APP recovered by the two acid washes should rapidly decline and remain at low levels.
Nature Struct. Biol. 6, 327, 1999. 25. Hilbich, C. et al. Amyloid-like properties of peptides flanking the epitope of amyloid precursor protein-specific monoclonal antibody 22C11. J. Biol. Chem. 268, 26571, 1993. 26. , Crystallization of Biological Macromolecules, Cold Spring Harbor Laboratory Press, New York, 1999. 27. M. et al. Amino-terminal region of the beta-amyloid precursor protein activates mitogen-activated protein kinase. Neurosci. Lett. 198, 52, 1995. 28. Iwasaki, W. et al. Solution structure of midkine, a new heparin-binding growth factor.
Amyloid precursor protein : a practical approach by Weiming Xia, Huaxi Xu